In our study, the regenerated structures initially had a globular
appearance (Fig. 1c and d). In addition to the small nodular
structures, first visual shoot organogenesis appeared on globular
structures in the following weeks. The oval response was similar
to that achieved in onion (Luthar and Bohanec, 1999), A. giganteum
(ˇSuˇsek et al., 2002). The results indicated that the best shoot
differentiation could be promoted by combining 9.05 M 2,4-D
and 8.88 M BAP. This observation was similar to the report that
flowers of onion and hybrids cultured on B5 induction medium containing
9.05 M 2,4-D and 8.88 M BAP gave a good organogenic