2. Material and methods
2.1 Plant material
Three-month-old capsule was collected from Doritis pulcherrima var. buyssoniana plants cultured in a shade house. The capsule was surface sterilized with 70% alcohol for 2 minutes and direct flame for 30 seconds. Seeds from the capsule were sown on modified Vacin and Went medium (VW). Young leaves, 1-2 cm. in length, were taken from six-monthold seedlings by cutting at their base and then crossly divided into two explants (basal and tip segments).
Micropropagation experiments were carried out with
these explants. A completely randomized design
(CRD) was used in all experiments