The objective of this study was to investigate the antioxidant
and anti-inflammatory effects of methanol, ethanol and water
extracts from O. aristatus (abbreviated as MEOA, EEOA and
WEOA, respectively). In addition, an aim was to determine the
bioactive compounds in the LPS-stimulated RAW 264.7 murine
macrophage cells. In the present work, MEOA, EEOA and
WEOA were prepared and evaluated for their antioxidant
activity by total phenolics, oxygen radical absorbance capacity
(ORAC), Trolox equivalent antioxidant capacity (TEAC) and
cellular antioxidant activity (CAA) assays. Moreover, we also
examined the effects ofMEOA, EEOAandWEOA, aswell as the
bioactive compounds on the generation of NO, PGE2 and ROS.
Furthermore, we examined the effect ofEEOAon the protein and
mRNAexpression of iNOS and COX-2 in LPS-stimulatedRAW
264.7 cells.
The objective of this study was to investigate the antioxidantand anti-inflammatory effects of methanol, ethanol and waterextracts from O. aristatus (abbreviated as MEOA, EEOA andWEOA, respectively). In addition, an aim was to determine thebioactive compounds in the LPS-stimulated RAW 264.7 murinemacrophage cells. In the present work, MEOA, EEOA andWEOA were prepared and evaluated for their antioxidantactivity by total phenolics, oxygen radical absorbance capacity(ORAC), Trolox equivalent antioxidant capacity (TEAC) andcellular antioxidant activity (CAA) assays. Moreover, we alsoexamined the effects ofMEOA, EEOAandWEOA, aswell as thebioactive compounds on the generation of NO, PGE2 and ROS.Furthermore, we examined the effect ofEEOAon the protein andmRNAexpression of iNOS and COX-2 in LPS-stimulatedRAW264.7 cells.
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