Banana production has been severely hindered by fusarium wilt disease. The application of organic
amendments to soil has been proposed as a strategy for management of the soil borne disease. The oneyear effects of different composts and bio-organic fertilizer (BIO) on banana fusarium wilt disease
incidence, crop yield, crop quality, and the soil microflora were investigated infield condition. A plate
count method, real-time PCR, and PCR-DGGE were used to investigate the soil microflora. The BIO
application more effectively controlled fusarium wilt disease. The highest total soluble sugars (TSS) to
titratable acidity (TSS/TA) ratios, banana yield, culturable and total soil bacteria populations, and culturable actinobacteria population were observed from the BIO treatment. The ratio of bacteria to fungi (B/
F) and actinomycetes to fungi (A/F) values from the plate count method and the B/F value from the realtime PCR analysis were also highest in the soils collected from the BIO application. Based on PCR-DGGE
results, the soil bacteria structure was significantly altered in the soils collected from BIO treatment
compared to the other treatments. All the results confirmed that application of bio-organic fertilizer
could more effectively control fusarium wilt disease in field conditions by improving soil microbial
communities
Banana production has been severely hindered by fusarium wilt disease. The application of organicamendments to soil has been proposed as a strategy for management of the soil borne disease. The oneyear effects of different composts and bio-organic fertilizer (BIO) on banana fusarium wilt diseaseincidence, crop yield, crop quality, and the soil microflora were investigated infield condition. A platecount method, real-time PCR, and PCR-DGGE were used to investigate the soil microflora. The BIOapplication more effectively controlled fusarium wilt disease. The highest total soluble sugars (TSS) totitratable acidity (TSS/TA) ratios, banana yield, culturable and total soil bacteria populations, and culturable actinobacteria population were observed from the BIO treatment. The ratio of bacteria to fungi (B/F) and actinomycetes to fungi (A/F) values from the plate count method and the B/F value from the realtime PCR analysis were also highest in the soils collected from the BIO application. Based on PCR-DGGEresults, the soil bacteria structure was significantly altered in the soils collected from BIO treatmentcompared to the other treatments. All the results confirmed that application of bio-organic fertilizercould more effectively control fusarium wilt disease in field conditions by improving soil microbialcommunities
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