Seed production started from the
4th week of broodfish stocking in tanks. Seed were collected in
plastic bowls from the mouths of incubating females as
described by Little et al. (1993) every Monday between 08:00
and 10:00 h. Total weights of each stage of eggs (stage I: just
spawned eggs, Stage II: eggs with two eye spots, stage III: with
protruding eyes and a tail, stage IV: yolk-sac larvae with well
developed head and tail) from each replicate tank were
measured with an electronic balance (up to two decimal points
of g). A towel and a small hand net were used to dry the water
from egg mass before weighing.