liter of distilled water under constant aeration and
light source (29
o
C) for 48 h. After hatching, the
larvae (active nauplii) were separated from the
shells and unhatched eggs by siphoning with a
plastic
tube. Ten nauplii were drawn through a
glass capillary and placed in a vial containing 4.5
ml of artificial sea water solution and 0.5 ml of