Organic manure and chemical fertilizers (N, P, K, S) are known to play a pivotal role in many production oriented agricultural systems, but little is known about the interactive effects of various chemical fertilizers on growth and secondary metabolites accumulation in medicinal plants. Artemisinin a sesquiterpene-lactone is synthesized through terpenoid pathway. Efficiency and production of these secondary metabolites depends on the rate of photosynthesis for their energy and carbon requirements (Singh et al., 1990). Hence, by enhancing the rate of photosynthesis through adequate supply of phytonutrients may increase the accumulation of artemisinin in A. annua L