Materials and Methods Collection of plant material: The different parts of Andrographis paniculata Burm.F such as leaf, root and stem were collected from the field grown plants in Botanical garden, Andhra University, Visakhapatnam, Andhra Pradesh and were collected for the following experiments. The plant materials were dried under shade with occasional shifting and then powdered with a mechanical grinder and stored in airtight container. The powder obtained was subjected to successive soxhlet extraction with the organic solvents with increasing order.