Regulation of VEGF production in ZFP36 siRNA-transfected cells after scratching. After scratching, VEGF concentrations in conditioned medium were determined byELISA analysis as indicated. The top graph represents the time course of VEGF levels in the conditioned medium of untransfected cells, incubated in fresh medium overnight(pre), and at the indicated time points after scratching. The bottom graphs show VEGF concentrations in the conditioned medium of siRNA-transfected cells, before scratching(left), and at defined time points after scratching (right). Data are expressed as mean +/− SD (n = 3). **: p < 0.01; ***: p < 0.001.