The compositional distribution of the raw biomass samples estimated by gravimetric method [1,2](Table 1) has total polysaccharide content of 62.56% (w/w) indicating sugarcane bagasse as a potentialfeedstock for the production of fuels and chemicals.Table 2 shows the pretreatment operating parametersfor the duplicated experimental runs and the corresponding fermentable (reducing) sugars production.Figs. 1e3 show the interactions of pretreatment operating parameters on reducing sugars (RS)yields. The surface plots showed effective range prediction for the optimum production of fermentablesugars. Fig. 4 shows the experimental data and the predicted values having minimal deviations.Table 3 shows the analysis of variance (ANOVA) description of generated regression model (Eqs. 1and 2) used for the adequate prediction of reducing sugars with the operating pretreatment parameters values.Fig. 5 shows the validated optimum pretreatment conditions (Time: 4.63 h, H2O2: 0.32%v/v, andTemperature: 100.23 C) and the optimum value of RS (153.75 mg/g).