It has been reported that ozone treatment is more effective when microorganisms are suspended in pure water or buffers containing less ozone demanding materials than in complex food systems composed of organic compounds (Cho et al., 2003). Ozone concentration in treated apple juice couldn't be determined by the indigo method (data not shown) because of the intrinsic color of juice, but residual ozone in distilled water could be analyzed. Even though we obtained results about residual ozone by using distilled water instead of apple juice, low concentrations of residual ozone may occur in apple juice due to various ozone consuming compounds compared to distilled water. There are organic compounds such as sugars, pectic substances, and antioxidants in apple cider and orange juice. These compounds may react with ozone (Kim et al., 1999; Williams et al., 2004). Antioxidant compounds like polyphenols, phenolic acids,flavonoids and ascorbic acid are ozone consuming materials having an effect on ozone chelation in
apple juice (Liao et al., 2007). Therefore, application of the indigo method in this study is considered reasonable for judging the concentration of residual ozone in apple juice by inference through results obtained from distilled water
It has been reported that ozone treatment is more effective when microorganisms are suspended in pure water or buffers containing less ozone demanding materials than in complex food systems composed of organic compounds (Cho et al., 2003). Ozone concentration in treated apple juice couldn't be determined by the indigo method (data not shown) because of the intrinsic color of juice, but residual ozone in distilled water could be analyzed. Even though we obtained results about residual ozone by using distilled water instead of apple juice, low concentrations of residual ozone may occur in apple juice due to various ozone consuming compounds compared to distilled water. There are organic compounds such as sugars, pectic substances, and antioxidants in apple cider and orange juice. These compounds may react with ozone (Kim et al., 1999; Williams et al., 2004). Antioxidant compounds like polyphenols, phenolic acids,flavonoids and ascorbic acid are ozone consuming materials having an effect on ozone chelation in
apple juice (Liao et al., 2007). Therefore, application of the indigo method in this study is considered reasonable for judging the concentration of residual ozone in apple juice by inference through results obtained from distilled water
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