This study describes the standardization of an efficient in vitro propagation and hardening
procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings
were germinated on half-strength 1/2 MS medium supplemented with 10 g·L-1sucrose, 5.5 g·Lagar
at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·L-1
TDZ, 0.1 mg·LNAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds were successfully rooted on MS medium supplemented with 0.1 mg·L-1
IBA and 0.05 mg·L NAA resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes 35-42 days for acclimatized plantlet production.