Microbial forensic specimens that are collected at biocrime and bioterrorism scenes include blood, tissue,
cloths containing biological fluids, swabs, water, soil, and aerosols. It is preferable that pathogens in such
specimens are alive and kept in a steady state. Specimens may be stored for a prolonged period before
analysis; therefore, it is important to understand the effect of the storage conditions on the pathogens
contained within the specimens. In this study, we prepared blood and bloodstain specimens containing
Gram-negative or -positive bacteria, stored the samples for 482 days under various conditions, and
measured viable bacterial counts and total bacterial contents in the samples. Viable bacteria were preserved
well in the samples stored at 30 and 80 C, but were diminished or undetectable in the
samples stored at 4 C and room temperature. The total bacterial content was maintained in the blood
samples stored at 30 and 80 C and in the bloodstain samples stored under all temperature conditions,
but decreased in the blood samples stored at 4 C and room temperature. This study showed that
the storage conditions affected viable bacteria and bacterial DNA and that freezing and drying were
significant for their long-term storage. We provide important information for the storage of microbial
forensic specimens.
Microbial forensic specimens that are collected at biocrime and bioterrorism scenes include blood, tissue,cloths containing biological fluids, swabs, water, soil, and aerosols. It is preferable that pathogens in suchspecimens are alive and kept in a steady state. Specimens may be stored for a prolonged period beforeanalysis; therefore, it is important to understand the effect of the storage conditions on the pathogenscontained within the specimens. In this study, we prepared blood and bloodstain specimens containingGram-negative or -positive bacteria, stored the samples for 482 days under various conditions, andmeasured viable bacterial counts and total bacterial contents in the samples. Viable bacteria were preservedwell in the samples stored at 30 and 80 C, but were diminished or undetectable in thesamples stored at 4 C and room temperature. The total bacterial content was maintained in the bloodsamples stored at 30 and 80 C and in the bloodstain samples stored under all temperature conditions,but decreased in the blood samples stored at 4 C and room temperature. This study showed thatthe storage conditions affected viable bacteria and bacterial DNA and that freezing and drying weresignificant for their long-term storage. We provide important information for the storage of microbialforensic specimens.
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