Immediately
after collection with heparin, each blood sample (approximately
5 mL) was centrifuged at 3,000 × g for 10 min at
4°C to obtain plasma, which was stored at -20°C until analysis.
At 94, 95, or 97 d of age (4 to 6 pigs in each line per
day), the littermates were killed by jugular exsanguination
after electronarcosis, and then digestive, urogenital, and respiratory
tracts were removed.
Immediatelyafter collection with heparin, each blood sample (approximately5 mL) was centrifuged at 3,000 × g for 10 min at4°C to obtain plasma, which was stored at -20°C until analysis.At 94, 95, or 97 d of age (4 to 6 pigs in each line perday), the littermates were killed by jugular exsanguinationafter electronarcosis, and then digestive, urogenital, and respiratorytracts were removed.
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