Continuous cultivation was carried out at the same culture condition
as the batch cultivation. It started by feeding the brine at a
suitable concentration with various dilution rates into the bioreactor
and drawing broth with peristaltic pumps after the system
reached a steady state (or after the batch culture of 24 h). During
cultivation at each dilution rate, pH and dissolved oxygen in
the broth were monitored and the broths were determined for
yeast growth, -carotene, sugar and acid after 10 medium volume
changes. Their kinetic parameters were also calculated. All experiments
were performed in triplicate.