abstract Techniquesforspeciesassaya

abstract Techniquesforspeciesassayarepotenttoolsforthesupervisionofmeatadulteration.Matsunagaetal. haveestablishedareliablemultiplexPCRmethodtoidentifychicken,beef,porkandmuttonspeciesin meatproducts.However,thismethodwasnotsensitiveenoughintheassayoffurther-processedmeats. Here,weattempted2strategies,semi-nestedmultiplexPCRandshorteningprimers,toenhancethe sensitivityofmultiplexPCR.Asthesemi-nestedmultiplexPCR,firstPCRwasperformedbyapairof commonprimers,andtheproductwasusedasthetemplateofsecondmultiplexPCR.Thismethod loweredthelimitofdetection(LOD)ofmultiplexPCRby3ordersofmagnitude,andeffectivelyidentified meatspeciesinfurther-processedfoods.TheLODofsemi-nestedmultiplexPCRreached1pgofDNAper reaction,whichwas10-foldlowerthanastandardizedReal-timePCRmethod.Incontrast,multiplexPCR usingtruncatedprimerscouldhardlymeetidenticalefficiencyondifferenttemplates,andfailedto improvethemethodsensitivity.Thesemi-nestedmultiplexPCRestablishedinthisstudywouldbe practicalinthecontrolofmeatadulteration,andcouldbenefitQCofmeatmanufacture. 2012ElsevierLtd.Allrightsreserved.

ผลลัพธ์ (ไทย) 1:

techniquesforspeciesassayarepotenttoolsforthesupervisionofmeatadulteration.matsunagaetal นามธรรม haveestablishedareliablemultiplexpcrmethodtoidentifychicken เนื้อ porkandmuttonspeciesin meatproducts.however, thismethodwasnotsensitiveenoughintheassayoffurther processedmeats- ที่นี่ weattempted2strategies, nestedmultiplexpcrandshorteningprimers กึ่ง toenhancethe sensitivityofmultiplexpcrasthesemi-nestedmultiplexpcr, firstpcrwasperformedbyapairof commonprimers, andtheproductwasusedasthetemplateofsecondmultiplexpcr.thismethod loweredthelimitofdetection (หลอด) ofmultiplexpcrby3ordersofmagnitude, andeffectivelyidentified ปฏิกิริยา meatspeciesinfurther-processedfoods.thelodofsemi-nestedmultiplexpcrreached1pgofdnaper, whichwas10-foldlowerthanastandardizedreal-timepcrmethod.incontrast,usingtruncatedprimerscouldhardlymeetidenticalefficiencyondifferenttemplates multiplexpcr, andfailedto practicalinthecontrolofmeatadulteration improvethemethodsensitivity.thesemi-nestedmultiplexpcrestablishedinthisstudywouldbe, andcouldbenefitqcofmeatmanufacture 2012elsevierltd.allrightsreserved

ผลลัพธ์ (ไทย) 2:

บทคัดย่อ Techniquesforspeciesassayarepotenttoolsforthesupervisionofmeatadulteration.Matsunagaetal haveestablishedareliablemultiplexPCRmethodtoidentifychicken เนื้อ porkandmuttonspeciesin meatproductsอย่างไรก็ตาม thismethodwasnotsensitiveenoughintheassayoffurther-processedmeats ที่นี่ weattempted2strategies ห้อง nestedmultiplexPCRandshorteningprimers, toenhancethe sensitivityofmultiplexPCRAsthesemi-nestedmultiplexPCR, firstPCRwasperformedbyapairof commonprimers,andtheproductwasusedasthetemplateofsecondmultiplexPCR.Thismethod loweredthelimitofdetection (ลอด) ofmultiplexPCRby3ordersofmagnitude, andeffectivelyidentified meatspeciesinfurther-processedfoodsปฏิกิริยา TheLODofsemi nestedmultiplexPCRreached1pgofDNAper, whichwas10-foldlowerthanastandardizedReal-timePCRmethod.Incontrastusingtruncatedprimerscouldhardlymeetidenticalefficiencyondifferenttemplates multiplexPCR, andfailedto improvethemethodsensitivityThesemi-nestedmultiplexPCRestablishedinthisstudywouldbe practicalinthecontrolofmeatadulteration, andcouldbenefitQCofmeatmanufacture 2012ElsevierLtd.Allrightsreserved

ผลลัพธ์ (ไทย) 3:

นามธรรม techniquesforspeciesassayarepotenttoolsforthesupervisionofmeatadulteration . matsunagaetal . porkandmuttonspeciesin เนื้อวัว haveestablishedareliablemultiplexpcrmethodtoidentifychicken meatproducts .อย่างไรก็ตาม thismethodwasnotsensitiveenoughintheassayoffurther - processedmeats ที่นี่ weattempted 2 กลยุทธ์แบบกึ่ง nestedmultiplexpcrandshorteningprimers sensitivityofmultiplexpcr toenhancethe .asthesemi - nestedmultiplexpcr commonprimers firstpcrwasperformedbyapairof andtheproductwasusedasthetemplateofsecondmultiplexpcr . thismethod loweredthelimitofdetection (ลอด) ofmultiplexpcrby 3 ordersofmagnitude andeffectivelyidentified meatspeciesinfurther - processedfoods . thelodofsemi - nestedmultiplexpcrreached 1 pgofdnaper ปฏิกริยา whichwas 10 - foldlowerthanastandardizedreal - timepcrmethod . incontrastusingtruncatedprimerscouldhardlymeetidenticalefficiencyondifferenttemplates multiplexpcr andcouldbenefitqcofmeatmanufacture practicalinthecontrolofmeatadulteration andfailedto improvethemethodsensitivity . thesemi - nestedmultiplexpcrestablishedinthisstudywouldbe . 2012 elsevierltd .สงวนลิขสิทธิ์.