A 26.9 g solution of urea (400 g/l) was addicted to bioreactor as nitrogen source. Total dry yeast (2,45 g) was
rehydrated in water (24,5 g) at 30 °C for 15 min and then inoculated. The fermentation was carried out for 48h and
samples were collected after 1, 3, 24 and 48h for ethanol concentration determination.