However, moisture content below the optimum level (too low moisture content) might reduce the nutrient solubility in the substrate and affect the initial growth of fungal spores (which could disrupt the fungal growth and enzyme activity) (Murthy et al., 1993; Battan et al., 2006). Low water content is usually related to insufficient substrate swelling which prevented the nutrient absorption from the substrates. Low moisture content not only lessened substrate swelling but also reduced nutrient solubility and caused higher water tension which also result poor fungal growth (Syarifah et al., 2011). On the other hand, higher moisture contents resulted reduction in substrate porosity and caused oxygen limitation within the substrates which consequently affected the oxygen transfer within the substrate and thus resulting poor growth (Ramesh and Lonsane, 1990; Sandhya et al., 2005).