Chlorella sp. IM-01 is inoculated in autoclaved wastewater samples (0.01 initial OD) without any other nutritional supplement and allowed to grow at light intensity of 2000 lux at 27 ± 3 °C temperature for 10 days test period. BG-11 liquid media without wastewater to be designated as control (C) was also inoculated with microalgae and experiments are run simultaneously.