solid mass (18.7% ± 0.88, methanol free) obtained was used for the in vitro biological activities and for the formulation of a 5% (w/w)
extract-based
ointment,
using
soft
white
paraffin,
vehicle (Fig. 1D).
HPLC-fingerprint
analysis
of
PgME
Sample preparation. The PgME was prepared to a concentration of
l. Before injection, PgME was centrifuged (4 min at 5000 rpm) and the centrifuged supernatant was filtered through a 0.45 m PTFE filter (Waters), and submitted to HPLC–DAD and HPLC–ESI–MS analysis.
12
mg/ml,
with
injection
volume
of
10
Apparatus. Shimadzu controller (SLC-10A vp) with of a vacuum degasser (DGU-14A), a binary pumps (LC-10AD vp), an oven (CTO10AC
vp)
and
a
Shimadzu
Diode
Array
Detector
The