A Gram-staining-positive, coccus-shaped, non-spore-forming, facultatively anaerobic bacterium,
designated AC-1
T
, was isolated from an acidogenic fermentation bioreactor treating food
wastewater. On the basis of 16S rRNA gene sequence analysis, strain AC-1
T
was shown to belong
to the genus Vagococcus. The closest phylogenetic relatives were Vagococcus elongatus PPC9
(97.4 % 16S rRNA gene sequence similarity), Vagococcus penaei CD276
(96.7 %) and
Vagococcus carniphilus ATCC BAA-640
T
(96.6 %). The major fatty acids were C
v9c
(24.8 %) and C
16 : 0
T
(19.5 %) and the G+C content of genomic DNA was 44.2 mol%, which
supported the affiliation of strain AC-1
T
to the genus Vagococcus. Strain AC-1
and V. elongatus
DSM 21480
T
exhibited 11 % DNA–DNA relatedness. Physiological and biochemical tests
differentiated strain AC-1
T
from the type strains of recognized species of the genus Vagococcus.
Therefore, strain AC-1
T
is considered to represent a novel species, for which the name Vagococcus
acidifermentans sp. nov. is proposed. The type strain is AC-1
T
(5KCTC 13418
T
T
18 : 1
5LMG 24798
T
T
).