Immediate mortality was observed in fairy shrimp after exposure to A. hydrophila WS1 and E. ludwigii WS5 in the 24-hour study period and the clinical signs of black disease were evident. Black nodules on the thoracic appendage and exoskeleton were observed. The fairy shrimp appeared weak and lethargic after exposure to Acinetobacter sp. WS2, Chryseobacterium sp. WS3 and C. youngae WS4 for 24 hours and experienced increased mortality after two days. The highest cumulative mortality rate was associated with fairy shrimp challenged with A. hydrophila WS1 (62.00 ± 5.70%), followed by E. ludwigii WS5, C. youngae WS4, Acinetobacter sp. WS2 and Chryseobacterium sp. WS3 (51.00 ± 4.18, 35.00 ± 3.54, 10.00 ± 3.54 and 9.00 ± 4.18%, respectively).