2.6. Measurement of total phenolic content
A 3-g slurry sample, as prepared above, was homogenized with 30 ml of 100% methanol, and total phenolic contents were determined with the Folin–Ciocalteu reagent using gallic acid as a standard (Slinkard & Singleton, 1977). Absorption was measured at 765 nm, and the results were expressed as mg gallic acid equivalence (GAE)/100 g of fresh weight.