2.2. Essential oil extraction
The essential oil from the semi-crushed seeds of C. paniculatus
was extracted using hydro-distillation. The semi-crushed seeds
(200 g) were packed in a Clevenger apparatus with 400 mL of water
and were submitted to hydro-distillation. The extraction was carried
out for 8 h. The isolated oil was collected from the graduated
receiver. For the removal of water traces from the oil, the extract
was treated with anhydrous sodium sulphate (Na2SO4). The oil
samples were stored in sealed vials in a refrigerator at 4 ◦C until
used for analysis. The yield of the oil was found to be 0.09% (v/w).