.2. Gluten washingIbis wheatflour (200 g) was mixed with 120 g of tap water andallowed to rest for 15 min. Then, the dough ball was gently knea-ded, placed in a 500-ml beaker full of tap water at ambient tem-perature, and allowed to stand for 5 min. This step was repeatedthree times. Afterwards, the remaining starch was continuouslywashed out under running tap water until the dough ball mass hadapproximately reduced to 16e18% of the initial mass. The doughball was then freeze-dried, ground, and used for hydrolysis. Theaverage crude protein and water content of the self-washed Ibisgluten were 86.6±1.1% and 1.9±0.2%, respectively