The fractions (8 mL/each tube) were collected at a flow rate of 4 mL/min and monitored using
the phenol-sulfuric acid method at 490 nm. After a process purification of the crude polysaccharides, the resin was regenerated by elution of 2 mol/L NaCl solution at a flow rate of 40 mL/min until the absorbance at 280 nm of the eluent dropped to the baseline,.