Bioassay for bacteria-induced trap formation in
A. oligospora
Trap formation was bioassayed using small Petri plates
(60 mm diameter). Two Arthrobotrys isolates were used:
A. oligospora (ATCC 24927) and A. oligospora (1.1495).
Tested solutions and controls (each 3 mL) were added to
Petri plates together with 200 mL of freshly harvested conidia
of A. oligospora and incubated at 25 1C. Traps were never
observed when conidia of A. oligospora were cultured only in
the negative control media for nearly 1 month. The Petri
plates were assessed 8, 16, 24 and 48 h after inoculation for
the presence of traps, using an inverted microscope. Approximately
100 conidia of A. oligospora were scored for trap
formation in each experiment.