Determination of chlorophyll contents
Chlorophyll was extracted by incubating each detached organ
with 20 mL of 80% acetone in test tubes at 55 ◦C for 2 h. Chlorophyll
contents were determined as OD values at 663 and 645 nm using a
Hitachi Gene Spec V spectrophotometer. Chlorophyll contents were
calculated using the equations from Porra et al. (1989).