The cryoprotective effects of trehalose, alginate, and its oligosaccharides on peeled shrimp (Litopenaeus
vannamei) during frozen storage was investigated by monitoring thawing loss, color, texture, myofibrillar protein content,
Ca2+-ATPase activity, and performing microscopic structural analysis. Data revealed significant (p < 0.05) inhibitory
effects on thawing loss and textural variables (springiness and chewiness) in trehalose-, alginate oligosaccharides-, and
sodium pyrophosphate-treated shrimp compared with the control and alginate-treated batches. L∗ values revealed that
these saccharides had a positive effect on color stability during frozen storage. In addition, the results of chemical
analyses showed that trehalose and alginate oligosaccharide treatments effectively maintained an increased myofibrillar
protein content and Ca2+-ATPase activity in frozen shrimp. In addition, hematoxylin & eosin staining and SDS-PAGE
confirmed that these cryoprotective saccharides slowed the degradation of muscle proteins and the damage to muscle
tissue structures. Overall, the application of trehalose and alginate oligosaccharides to peeled frozen shrimp might maintain
better quality and extend the commercialization of these refrigerated products.