2. Materials and Methods
2.1. Animals
Adult male albino rats of Wistar strain weighing about 200–
250 g were used for the in vivo experiments. Adult female albino
rats of Wistar strain weighing 150–200 g were used for the
in vitro experiments. Animals were obtained from the National
Research Center, Cairo, Egypt. The animals were housed in
plastic cages (28 cm · 43 cm · 18 cm) and were maintained under
conventional laboratory conditions throughout the study.
They were fed standard pellet chow (El-Nasr Chemical Co.,
Cairo, Egypt) and were allowed water ad libitum. Animals described
as fasted had been deprived of food for 18 h but had been
allowed free access to water. This design is accepted by the ethics
committee in the Faculty of Pharmacy Beni Sueif University