than buffer alone at identical pH in dissociating
Abs from Protein-A. For example, at pH 4.3, 0.1–
0.5 M citrate is almost ineffective while 0.5–2 M
arginine can effectively elute Abs, as shown in
Figure 7.33,37 Citrate above 0.5 M was totally
ineffective in eluting mAb from Protein-A at pH 4.3
due to its salting-out effect occurring at high
concentrations. Arginine at 1 and 2 M above pH
4.3 efficiently eluted Abs (Fig. 7)