Quantitative PCR was performed in order to assay FOL2 and ABZ1 overexpression. Two days after the start of fermentation, aliquots of yeast cells were withdrawn, and total RNA prepared using an RNEasy kit according to the manufacturer’s instructions
Quantitative PCR was performed in order to assay FOL2 and ABZ1overexpression. Two days after the start of fermentation, aliquotsof yeast cells were withdrawn, and total RNA prepared using anRNEasy kit according to the manufacturer’s instructions