Internal standard method
The highest precision for quantitative chromatography is obtained by using internal standards because the uncertainties introduced by sample injection, flow rate, and variations in column conditions are minimized. In this procedure, a carefully measured quantity of an internal standard is introduced into each standard and sample, and the ratio of analyte peak area (or height) to internalstandard peak area (or height) is the analytical parameter. For this method to be successful, it is necessary that the internal-standard peak be well separated from the peaks of all other components in the sample, but it must appear close to the analyte peak. With a suitable internal standard, precisions of 0.5-1% relative are reported.
Internal standard methodThe highest precision for quantitative chromatography is obtained by using internal standards because the uncertainties introduced by sample injection, flow rate, and variations in column conditions are minimized. In this procedure, a carefully measured quantity of an internal standard is introduced into each standard and sample, and the ratio of analyte peak area (or height) to internalstandard peak area (or height) is the analytical parameter. For this method to be successful, it is necessary that the internal-standard peak be well separated from the peaks of all other components in the sample, but it must appear close to the analyte peak. With a suitable internal standard, precisions of 0.5-1% relative are reported.
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