0.50 g/L biomass. Fermentations were performed during 144 h in
200 mL bottles placed in oven at 25, 28, 30 and 35
C.
Studies for the selection of initial sucrose concentration were
performed on the selected strains during 120 h by triplicate in
200 mL bottles containing 50 mL YPS fermentation medium,
changing the initial amount of sucrose to 250, 300, or 350 g/L.
Flasks were inoculated with 0.5 g/L of biomass and incubated in
oven at 30
C.