ChonbukNational University Laboratory Animal Center. BALB/c mice (n ¼ 4) were
sensitized by i.p. injection of 50 mg ovalbumin (OVA, SigmaeAldrich) on day 1. On
day 8 and 15 mice were challenged intranasally with 50 mg of OVA.Mice were given
50 mL of POX or HPOC nanoparticles intranasally on days 16 and 17. Control mice
were given PBS only. On day 18, mice were euthanized and their lungs were
perfused with PBS, removed and fixed in 4% buffered formalin. Lungs were
embedded in paraffin, sectioned and stained with hematoxylin and eosin.
Unstained lung tissue sections were also stained with a monoclonal antibody for
iNOS (Santa Cruz, US)