The enzyme oxidizes the hydroxyl group to give an aldehyde group plus hydrogen peroxide, which in turn, in the presence of peroxidase, oxidizes a chromogen group whose colour is measured spectrophotrometrically.
The enzyme oxidizes the hydroxyl group to give an aldehydegroup plus hydrogen peroxide, which in turn, in thepresence of peroxidase, oxidizes a chromogen groupwhose colour is measured spectrophotrometrically.