An intracellular esterase/lipase
was reported from Bacillus circulans [9]. Recently, we have
reported a low molecular weight (31kDa) extra-cellular alkaline
lipase of a thermophilic B. coagulans BTS-3 strain [10]. For
newly isolated microbes, it is essential to characterize their
enzyme(s) if they are intended for use in biotechnological application(s). In the present study, we are reporting puriWcation,
biochemical characterization, and a few important
properties of an extra-cellular alkaline thermotolerant
lipase of B. coagulans MTCC-6375.