The shed dried leaf skin of A. vera were powdered in a mixer grinder (Champ Essentials, Morphy Richards, India). Leaf skin (0.1 g each from in situ and in vitro grown plants) were separately extracted with methanol (2× 20 ml) for 15 min under reflux on a water bath at 70 °C. The extracts were filtered through Whatman no: 1 filter paper (separately for in situ and in vitro samples) and were evaporated under vacuum using a rotary evaporator (Eyela, N-1100, China) to furnish a solid mass of extract.