a b s t r a c t
Our objective was to evaluate the antioxidant activity of olive leave extract (OLE) encapsulated by
nano-emulsions in soybean oil. The average droplet size one day after production was 6.16 nm for primary
W/O nano-emulsion and, 675 nm and 1443 nm for multiple emulsions stabilized by WPC alone
and complex of WPC–pectin, respectively. The antioxidant activity of these emulsions containing three
concentrations of 100, 200 and 300 mg OLE during storage was evaluated in soybean oil by peroxide
value, TBA value and rancimat thermal stability test and was compared with blank (non-encapsulated)
OLE and synthetic TBHQ antioxidant. Nano-encapsulated OLE was capable of controlling peroxide value
better than unencapsulated OLE. But because of blocking phenolic compounds within dispersed emulsions
droplets, thermal stability of encapsulated OLE was lower. To summarize, with increased solubility
and controlled release of olive leaf phenolic compounds through their nano-encapsulation, a higher
antioxidant activity was achieved.