Purified Ara h 2 from differentially thermal-treated peanuts was applied to study the impact of thermal processing on the allergenicity of Ara h 2 in mine. The immunization procedure is shown in Fig 4A. Typical allergy symptoms, including increased vascular permeability, spleen swelling, and mast cell degranulation (Fig 4B), were observed after intraperitoneal injection of crude Ara h 2, indicating that Balb/c mice were sensitized by the Ara h 2 allergen.