2.6. Hydroxyl radical scavenging activity
The hydroxyl radical scavenging activity was determined
according to the method of Klein, Cohen, and Cederbaum
(1991). Various concentrations (50, 100 and 200 ppm) of
conserves in ethanol were placed in different test tubes
and evaporated. One ml of iron–EDTA solution (0.13%
ferrous ammonium sulfate and 0.26% EDTA), 0.5 ml of
EDTA (0.018%), and 1 ml of DMSO (0.85% v/v in 0.1 M
phosphate buffer, pH 7.4) were added to these tubes and
the reaction was initiated by adding 0.5 ml of 0.22% ascorbic
acid. Test tubes were capped tightly and heated on a
water bath at 80–90 C for 15 min. The reaction was terminated
by the addition of ice-cold trichloroacetic acid
(17.5% w/v)