Leaf colour and greenish characteristics
Under various temperature, significant changes in seedling leaf color were observed, which showed that 'W25' is a temperature-sensitive mutant. At temperatures below 25°C. the leaves of'W25' were completely albino at the 1-2 leaf stage, and yellow-green or normal green at temperature above 30°C. When the mutant was grown at a temperature above 25°C, a greenish colour was visible at the 3 leaf stage, the color at the sixth leaf was a normal green. but the eighth leaf partly reverted to albinism. The leaves gradually turned green and the plant went through the whole growth cycle; while at a temperature below 25°C, the mutant seedling did not turn green and died at the 3 leaf stage. This indicated that it was a conditional lethal mutant. The parent '2177s' maintained a normal green colour under all tested conditions. When seedlings grown on 0.7% agarose or 0.7% agarose with 5 g/1 sucrose at 23°C, all leaves showed a light green colour at the 1-2 leaf stage, and at 25°C they were light green or green. The process of greening at the 3-6 leaf stage could be shortened obviously when 100 ppm of vitamin B, or B6 was applied to the seedling. However, there was no reaction to vitamins when sprayed at the 9-12 leaf stage.
Leaf colour and greenish characteristics Under various temperature, significant changes in seedling leaf color were observed, which showed that 'W25' is a temperature-sensitive mutant. At temperatures below 25°C. the leaves of'W25' were completely albino at the 1-2 leaf stage, and yellow-green or normal green at temperature above 30°C. When the mutant was grown at a temperature above 25°C, a greenish colour was visible at the 3 leaf stage, the color at the sixth leaf was a normal green. but the eighth leaf partly reverted to albinism. The leaves gradually turned green and the plant went through the whole growth cycle; while at a temperature below 25°C, the mutant seedling did not turn green and died at the 3 leaf stage. This indicated that it was a conditional lethal mutant. The parent '2177s' maintained a normal green colour under all tested conditions. When seedlings grown on 0.7% agarose or 0.7% agarose with 5 g/1 sucrose at 23°C, all leaves showed a light green colour at the 1-2 leaf stage, and at 25°C they were light green or green. The process of greening at the 3-6 leaf stage could be shortened obviously when 100 ppm of vitamin B, or B6 was applied to the seedling. However, there was no reaction to vitamins when sprayed at the 9-12 leaf stage.
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