2.1.1.1. Aerobic-dark conditions (Chemoorganotroph). 1 mL
inoculum was added to a screw cap test tube (150 25 mm:
50 mL) containing 9 mL GA medium with 0.25% NaCl (w/v), and all
culture tubes were incubated in a shaker at 150 rpm and 30 C, for
48 h. Dark culture conditions were ensured by covering the tubes
with aluminum foil, impenetrable by light. Bacterial growth was
measured using a spectrophotometer as previously described, andblanks with the medium but without inoculation were used as
control samples.