3.2. Continuous fermentation
Continuous ethanol fermentation using TSC-cell culture in a 1 L
packed-bed reactor was carried out at the following operating conditions:
32 ± 1 C, feed sugar concentrations of 220 and 240 g/L and
dilution rates of 0.04, 0.15, 0.24 and 0.36 h1. The steady-state ethanol
concentration and productivity are shown in Fig. 4 as functions
of the dilution rate. Table 2 summarizes the cell concentrations
after 20 days of continuous operation along with the average
immobilized yield (YI) and the average ethanol yield (YP/S).