The detection limits for meat species stored at 80 C for
13 weeks were 1 ng cDNA (Fig. 4). During storage at 80 C, the
meat started to brown from the 8th to 13th week, but without corruption.
Notably, the ostrich DNA PCR fragment was clearly
observed from 1 to 9 weeks of storage using 1 ng of cDNA template,
but the band intensity was weak from 10 to 13 weeks
(Fig 4E, lane 1–9). These results showed that 100 ng of cDNA
template was required for ostrich detection (data not shown).
Altogether, these results suggest that the detection limit was 10–100 ng cDNA template for meat stored at 4 C for 10 days and
1–100 ng cDNA template for meat stored at 80 C for 13 weeks.
There are few studies concerning the changes in adulterated meat
characteristics during storage. In the present study, the adulterated
meats were specifically and efficiently verified at different storage
times under refrigeration.