The selective hydrolysis with endo--mannanase of roasted
SCG mannans followed by the separation of the released oligosaccharides
by size exclusion chromatography, and their analysis by
ESI-MS/MS shows that the roasting process at 160 ◦C followed
by 4 M NaOH extractions at 20 ◦C, 60 ◦C, and 120 ◦C applied to
SCG improves the extractability of galactomannans without their
apparent degradation, preserving their -(1→4)-Man backbone,
Gal and Ara side chains, and acetylation.