The aim of these experiments was to identify an ethanol concentration having a clear influence in the growth kinetics of the
original yeast strains (i.e. constituting a selective pressure), while still maintaining a dilution rate that would allow to get hundreds of generations in just a fewweeks. The strainswere cultivated in YPD5 medium supplemented with different
amounts of ethanol, and growth kinetics was monitored as described in Materials and Methods.