Among the phosphate sources used, only K2HPO4 was significant at 99.83% and there was considerable difference in lactic acid production among the two phosphate sources used. Highest production was achieved with K2HPO4 and thus it was selected for further studies. Although FeSO4 · 7H2O was significant at 97.22% and had a negative effect on lactic acid production, considering the lactic acid production, it was better than other compounds having negative effect and thus its lower concentration was used for further optimization studies.