Based on design of the present experiment, we were unable to conclude whether the use of infected semen for AI of superovulated uninfected donors had a detrimental effect on the number of retrieved embryos or their development. This would have required use of “clean” semen from the same persistently infected bull for AI. Nevertheless, the mean number of transfer-quality embryos collected in our study was lower than that reported by other authors for superovulated heifers inseminated with uninfected semen [37].