The highest amount of reducing sugar was obtained at 40 °C which falls within the range for optimum activity of the enzymes. It was at enzyme concentration of 1.0% v/v that the enzyme produced its maximum reducing sugar. Though enzyme activity increases with increase in enzyme concentration, when a peak is reached, addition of more enzyme does not necessarily increase to the activity of the enzyme.
The reaction time also affects the hydrolysis of cassava starch in that it was observed after 24 h of hydrolysis that there was an increase in the concentration of reducing sugars which could probably be as a result of a reversion process. The reversion process involves the conversion of glucose to iso-maltose by amylo-glucosidase, when there is high concentration of glucose in the hydrolysate (Padmanabham and Losame, 1993), as may be the case in cassava starch that appeared to be more readily hydrolyzed, probably because it contains different proportions of low molecular weight carbohydrates.